BMSCs differentiated into neurons, astrocytes and oligodendrocytes alleviated the inflammation and demyelination of EAE mice models

Multiple sclerosis (MS) is a complex, progressive neuroinflammatory disease associated with autoimmunity. Currently, effective therapeutic strategy was poorly found in MS. Experimental autoimmune encephalomyelitis (EAE) widely used to study the pathogenesis of Cumulative research have shown that bone marrow mesenchymal stem Cells (BMSCs) transplantation could treat EAE animal models, but mechanism divergent. Here, we systematically evaluated whether BMSCs can differentiate into neurons, astrocytes and oligodendrocytes alleviate symptoms mice. We Immunofluorescence staining detect MAP-2, GFAP, MBP evaluate oligodendrocytes. The effect on inflammatory infiltration demyelination mice were detected by Hematoxylin-Eosin (H&E) Luxol Fast Blue (LFB) staining, respectively. Inflammatory factors expression ELISA RT-qPCR, Our results showed be induced neuron cells, oligodendrocyte vivo vitro , transplanted easier than normal Moreover, reduced neurological function scores incidence alleviated inflammation Finally, down-regulated levels pro-inflammatory TNF-α, IL-1β IFN-γ, up-regulated anti-inflammatory IL-10 TGF-β. In conclusion, this response mice, which may achieved differentiation